Analysis of the Promoter of the Autographa californica Nuclear Polyhedrosis Virus p10 Gene
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چکیده
منابع مشابه
Analysis of the promoter of the Autographa californica nuclear polyhedrosis virus p10 gene.
Functional analyses of the p10 gene promoter from the Autographa californica nuclear polyhedrosis virus (AcNPV) were performed by progressively deleting the 230 nucleotides upstream from the p10 coding sequences towards the ATG codon. Truncated promoter sequences retaining the full 5' non-coding leader of p10 were inserted in front of the chloramphenicol acetyltransferase (CAT) gene, and promot...
متن کاملA baculovirus vector derived from immediately early gene promoter of Autographa californica nuclear polyhedrosis virus.
A transfer vector was constructed in which the neomycin resistance (neo) gene was under the control of a copy of Autographa californica nuclear polyhedrosis virus (AcMNPV) IE1 gene promoter at the p10 locus. After cotransfection of Spodoptera frugiperda (Sf9) cells with the transfer vector and infectious AcMNPV DNA, the recombinant virus-containing neo gene was selected by serial passage of the...
متن کاملThe pk-1 gene of Autographa californica multinucleocapsid nuclear polyhedrosis virus encodes a protein kinase.
Open reading frame (ORF) 9 of the EcoRI I fragment of the baculovirus Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV) genome encodes a protein, PK-1, which has strong similarity to serine-threonine protein kinases. The published sequence of the pk-1 gene contains an error that, when corrected, extends the ORF by 228 nucleotides. Transcription of pk-1 was first detec...
متن کاملIdentifying the RNA polymerases that synthesize specific transcripts of the Autographa californica nuclear polyhedrosis virus.
Nuclear run-on assays carried out in the presence and absence of the RNA polymerase II inhibitor, alpha-amanitin, were used to determine the exact timing of the switch from inhibitor-sensitive transcription catalysed by host RNA polymerase II, to inhibitor-resistant transcription catalysed by the baculovirus-induced RNA polymerase. These studies revealed that the onset of alpha-amanitin-resista...
متن کاملTime Course Analysis and Mapping of Autographa californica Nuclear Polyhedrosis Virus Transcripts.
To study the expression of the Autographa californica nuclear polyhedrosis virus (AcNPV) genome, intracellular virus-specific proteins and mRNAs were pulsed-labeled, extracted, and analyzed at 6-h intervals during the replicative cycle. Most RNAs were detected between 12 and 24 h postinfection (p.i.), but many continued to be synthesized until late in infection. Polyhedrin and p8 mRNAs were the...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1989
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-70-1-203